Analytical Methods: Biomarkers And Molecular Biology

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Classification: Research, Research Type, Clinical, Research Practice, Research Design, Analytical Methods
based on the book chapter by Alpdogan Kantarci Summary The focus of this chapter is on the range of biomarkers used currently in orodental research at both laboratory and chairside levels. With definitions of what they are, the many different types (ranging from immune factors and hormones to genetic material of disease-causing bacteria and patients) and how they relate to surrogate markers and determinants, it explains how they provide dynamic, specific and accurate measures of disease status, that can be used to supplement information gained from conventional analytical methods. However, the chapter points out their enormous potential for predicting and diagnosing disease, for profiling patient risk, identifying deviations from healthy (before clinical manifestations occur), improving clinical decision-making and facilitating personalized therapy. Drawing on studies of wound healing and regenerative therapies, the authors describe various molecular methods including immunoassays, mass spectrometry, cell cultures, PCR-based assays and newer breakthroughs such as checkerboard hybridization. They review current areas of research into costs, processing time, and normal levels in various tissues (e.g. crevicular fluid, plaque and saliva) in addition to how they vary with individual factors such as gender, ethnicity and age. In clinical trials, cross-sectional longitudinal study designs and integration of clinical and laboratory data are recommended to deal with the complexities of orodental disease. Open full-text PDF (1.2 MB)
Osteology Foundation
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Osteology Foundation