Analytical Methods: Histology
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Classification: Research, Research Type, Clinical, Research Practice, Research Design, Analytical Methods
Content
based on the book chapter by Dieter D. Bosshardt Summary Histological assessment, which can be quantitative or qualitative, is an indispensable tool for investigation of soft tissue regeneration. Harvesting tissues raises ethical considerations, but less invasive forms of evaluation, such as clinical measures and imaging, only complement the descriptive analysis, rather than replace it, by showing how cells respond to biomaterials in vivo. This chapter explores various methods for evaluating hard and soft oral tissues, such as histomorphometry, in situ hybridization and autoradiography, in the context of procedures like socket preservation, ridge augmentation and sinus floor elevation. As well as outlining suitable endpoints and landmarks, it discusses tools and techniques for taking biopsies, cutting, sectioning and embedding samples, and the use of microscopy and illumination. The authors address the challenges posed by different biomaterials -- not all can be sectioned, and some react with the chemicals used for processing samples. Thus tissue sections may be of unpredictable quality and be affected by artifacts introduced before fixation (if the tissue gets crushed during harvesting or contaminated by oral pathogens) or during fixation or preserving. Other challenges include the acquisition, processing, analysis and storage of digital images, and the huge variations in analytical equipment and methods used in different laboratories, where the need to apply laboratory standards and minimize examiner variation is emphasized. Open full-text PDF (1.9 MB)
Details
Osteology Foundation
Content Type:
Document
Publication date:
05/02/2016
Publisher:
Osteology Foundation